A Test of Six Commercial 125 I-Labelled Digoxin Radioimmunoassay Kits
نویسندگان
چکیده
منابع مشابه
Some factors affecting a commercial kit for radioimmunoassay of digoxin using tritiated digoxin.
Some factors affecting results of digoxin determinations using one commercially available radioimmunoassay kit are described and discussed. Serum of pregnant women, cord blood, amniotic fluid and serum of patients taking spironolactone may show erroneously high digoxin activity due to lack of specificity of the antiserum. Cross-reaction with digitoxin was found to vary substantially with antibo...
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Samples from 33 patients being treated with digoxin and three concentrations of control material were analyzed for this drug by use of seven radioimmunoassay (RIA) kits and an automated fluorescence polarization immunoassay (FPIA) system. CVs ranged from 2.4 to 6.4% for the FPIA system and from 4.6 to 7.4% for two RIA methods. In the analysis of between-method variability of RIA kits, CVs range...
متن کاملMicroencapsulated antibodies in radioimmunoassay--I. Determination of digoxin.
We describe the application of the microencapsulated-antibody technique to the radioimmunoassay of digoxin in serum. Droplets of emulsified rabbit antibody are microencapsulated in a semipermeable nylon membrane by an interfacial polymerization technique. The antibody microcapsules are incubated with 125I-labeled digoxin and unlabeled digoxin for 15 min at 37 degrees C, then free and bound digo...
متن کاملVariability among commercially available digoxin radioimmunoassay kits in cross reactivity to dihydrodigoxin.
We evaluated four commercially available 125I-digoxin radioimmunoassay kits with regard to their ability to cross react with the digoxin metabolite dehydrodigoxin. We prepared dihydrodigoxin serum samples in digoxin-free serum over the concentration range 0.4 to 5.0 microgram/liter and assayed them with each kit according to the manufacturer's instructions. The metabolite was able to displace t...
متن کاملMeasurement of digoxin by radioimmunoassay.
A sensitive, specific and precise procedure for the measurement of digoxin by radioimmunoassay is presented. The method is rapid, convenient and highly reliable for this very important measurement. Studies designed to evaluate the validity and reproducibility of the assay are presented and discussed.
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1977
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1977.15.1-12.679